[Zurück]

C. Wagner, M. Schleeger, M. Vellekoop, J. Heberle, B. Lendl:
"Light induced time resolved Flow-Flash FTIR investigation in micro mixing cells";
Poster: International Conference on Advanced Vibrational Spectrosopcy, ICVAS 5, Melbourne; 12.07.2009 - 17.07.2009.

In this work we present our latest developments for step scan FTIR measurements to widen its applicability to noncyclic reactions. With our approach the
reaction between two chemicals can be monitored as well with a time resolution in the μs time range.
We are using a micro mixing device [1, 2] to pump the sample solution through the measurement spot of a specially designed focusing unit. The reaction in the mixer
is triggered by a laser flash and the spectrometer starts measuring the respective timeslice shortly before the laser flash hits the sample. While the measurement takes
place the sample is continously flowing. The flow rate is set in such a way that only a small portion of the sample volume is exchanged during the measurement. After
the measurement of the timeslice the whole volume is purged and the next laser pulse is fired onto a completley fresh sample in order to measure the next timeslice.
A typical step-scan experiment consumes only a few 10 μl of a mM sample solution, rendering it useful especially for biological samples.
We applied our technique to the photodissociation of the CO-myoglobin complex and followed the rebinding of CO including changes in the protein.

http://publik.tuwien.ac.at/files/PubDat_181339.pdf