Publications in Scientific Journals:
A. Reismann, L. Atanasova, S. Zeilinger, G. Schütz:
"Single-Molecule Localization Microscopy to Study Protein Organization in the Filamentous Fungus Trichoderma atroviride";
Molecules,
25
(2020),
319901
- 319909.
English abstract:
Abstract:Single-molecule localization microscopy has boosted our understanding of biologicalsamples by offering access to subdiffraction resolution using fluorescence microscopy methods.While in standard mammalian cells this approach has found wide-spread use, its application tofilamentous fungi has been scarce. This is mainly due to experimental challenges that lead to highamounts of background signal because of ample autofluorescence. Here, we report the optimization oflabeling, imaging and data analysis protocols to yield the first single-molecule localization microscopyimages of the filamentous fungusTrichoderma atroviride. As an example, we show the spatialdistribution of the Sur7 tetraspanin-family protein Sfp2 required for hyphal growth and cell wallstability in this mycoparasitic fungus.
Keywords:
SMLM; dSTORM; filamentous fungi;Trichoderma; superresolution microscopy
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.3390/molecules25143199
Electronic version of the publication:
https://doi.org/10.3390/molecules25143199