Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):
B. Devcic-Kuhar, K. Sankovic, J. Petrik, M. Gröschl, E. Benes:
"A novel method for detection of sonoporation";
Vortrag: 3rd Congress of the Alps Adria Acoustics Association,
Hotel Weitzer, Graz;
27.09.2007; in: "3rd Congress of the Alps Adria Acoustics Association, Hrsg: Joanneum Research",
(2007),
3 S.
Kurzfassung englisch:
One of the crucial problems of pharmacological therapy is a very low permeability of cell membranes. Pharmacological
compounds penetrate intact cell membranes by diffusion only poorly and may require specific carrier.
Ultrasound has the ability to change the permeability of cell membranes and to accelerate transport of drugs, genes,
and other therapeutic compounds through them. Detectability of transferred substances is one of the major issues in
evaluation of sonoporation. We have established an entirely new method for detection of biologically inert particles
within the cells. By seeding paramagnetic particles in extra-cellular space and by detecting their entrance into the cells
with Electron Paramagnetic Resonance Spectroscopy, it is possible to determine the uptake of the particles by the cells
due to sonication. At the present stage of our research, 1 nm paramagnetic particles (Magnevist; Schering, Germany)
were successfully delivered into the LLC-PK1 kidney cells by sonoporation.
Schlagworte:
Sonoporation, permeability, cell membranes, pharmacological therapy
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.