Vorträge und Posterpräsentationen (ohne Tagungsband-Eintrag):
N. Kaun, J. Frank, P. Svasek, M. Harasek:
"Time-Resolved FTIR Spectroscopy of Bioligand Interaction Using a Micro Fluidic Mixing System";
Poster: ICAVS II,
Nottingham (UK);
19.08.2003
- 24.08.2003.
Kurzfassung englisch:
This presentation deals with fast and highly reproducible diffusion-based mixing performed in a miniaturized flow cell for IR transmission measurements. A special design for the mixer has been developed in order to obtain fast mixing being subjected to the diffusion coefficient. At the same time the sample consumption is kept low. By mixing two reactants, e.g. an Enzyme and its inhibitor, the dynamic change in structure can be observed using time-resolved IR spectroscopy. This provides information about the connection between structure and function in complex biochemical systems. In this way e.g. the binding of vancomycin to cell wall analogues has already been successfully studied. The observation of the reaction process can be performed either in the stopped-flow or in the continuous-flow mode. A comparison of the two operation modes will be given by discussing experimental results along with simulation of fluid flow using computational fluid simulations.
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.