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B. Lendl:
"Stopped flow time resolved Fourier transform infrared spectroscopy for bio-ligand interaction studies";
Vortrag: Advanced Spectroscopies on Biomedical and Nanostructured Systems, Cluj-Napoca (eingeladen); 19.09.2004 - 22.09.2004.

Time resolved Fourier transform infrared (TR-FTIR) spectrometry was used to elucidate the binding of vancomycin - a glycopeptide antibiotic - to a cell wall precursor peptide with a time resolution in the low millisecond range. Using an IR transparent micro-machined mixer operated in the stopped flow mode superposition of two stream lines with a cross section of 7*1000 µm is achieved. For the performed bio-ligand interaction study vancomycin is introduced via one streamline and the glycopeptide antibiotic via the other. In this way rapid diffusion based mixing can be realized and the thus induced chemical reaction monitored in-situ by mid-IR spectrometry elucidating different kinetics of different binding centres involved.