[Zurück]

I Surowiec, J Baena, J. Frank, T. Laurell, J. Nilsson, M. Trojanowicz, B. Lendl:
"Flow-through microdispenser for interfacing µ-HPLC to Raman and mid-IR spectroscopic detection";
Journal of Chromatography A, 1080 (2005), S. 132 - 139.

A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier
transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets
can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid
and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy
they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have
been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition
infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with
the reference spectra of corresponding components.

http://aleph.ub.tuwien.ac.at/F?base=tuw01&func=find-c&ccl_term=AC05937046

http://publik.tuwien.ac.at/files/pub-tch_4814.pdf