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S. Kulka, G. Quintas, B. Lendl:
"Automated sample preparation and analysis using a sequential-injection-capillary electrophoresis (SI-CE) interface";
Analyst, 131 (2006), 6; S. 739 - 744.

A fully automated sequential-injection-capillary electrophoresis (SI-CE) system was developed using com. available components as the syringe pump, the selection and injection valves and the high voltage power supply. The interface connecting the SI with the CE unit consisted of two T-pieces, where the capillary was inserted in one T-piece and a Pt electrode in the other (grounded) T-piece. By pressurising the whole system using a syringe pump, hydrodynamic injection was feasible. For characterization, the system was applied to a mixt. of adenosine and adenosine monophosphate at different concns. The calibration curve obtained gave a detection limit of 0.5 mg g-1 (correlation coeff. of 0.997). The reproducibility of the injection was also assessed, resulting in a RSD value (5 injections) of 5.4%. The total time of anal., from injection, conditioning and sepn. to cleaning the capillary again was 15 min. In another application, employing the full power of the automated SIA-CE system, myoglobin was mixed directly using the flow system with different concns. of sodium dodecyl sulfate (SDS), a known denaturing agent. The different conformations obtained in this way were analyzed with the CE system and a distinct shift in migration time and decreasing of the native peak of myoglobin (Mb) could be obsd. The protein samples prepd. were also analyzed with off-line IR spectroscopy (IR), confirming these results.