[Zurück]

G. Quintas, E Nunez, M. Vellekoop, B. Lendl:
"On-line monitoring of pH junctions in capillary electrophoresis using Fourier transform infrared spectrometry";
Analytical and Bioanalytical Chemistry, 387 (2007), S. 287 - 292.

Fourier transform mid-infrared spectroscopic detection
is proposed as an on-line detection technique for the
study of on-line preconcentration processes in capillary
electrophoresis (CE). The molecule-specific information
contained in mid-IR spectra can be used to directly determine
the chemical compositions of individual zones and their
boundaries. This paper reports on pH junctions employed in
myoglobin analysis. On-line mid-IR detection allowed the
shape of the sample peak to be monitored as well as the
chemical compositions of the surrounding zones. From this
information it was possible to obtain detailed insights into the
actual chemical compositions of the individual zones governing
the efficiency of the preconcentration technique applied.
The principle of measurement outlined here can therefore also
be regarded as a promising one for investigating other on-line
preconcentration techniques, like stacking, sweeping, and pH
junction-sweeping among others.

http://dx.doi.org/10.1007/s00216-006-0944-x