[Zurück]

B.O. Petersen, M. Sára, C. Mader, H. Mayer, U. Sleytr, M. Pabst, M. Puchberger, E. Krause, A. Hofinger, J.Ø. Duus, P. Kosma:
"Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2";
Carbohydrate Research, 343 (2008), 8; S. 1346 - 1358.

The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-mannose, and 2-acetamido-2-deoxy-D-mannuronic acid. The primary structure of the acid-degraded polysaccharide - liberated by HF-treatment from the cell wall - was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-*-D-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-D-glucopyranosyl units.

http://dx.doi.org/10.1016/j.carres.2008.03.029