Publications in Scientific Journals:
B.O. Petersen, M. Sára, C. Mader, H. Mayer, U. Sleytr, M. Pabst, M. Puchberger, E. Krause, A. Hofinger, J.Ø. Duus, P. Kosma:
"Structural characterization of the acid-degraded secondary cell wall polymer of Geobacillus stearothermophilus PV72/p2";
Carbohydrate Research,
343
(2008),
8;
1346
- 1358.
English abstract:
The secondary cell wall polymer (SCWP) from Geobacillus stearothermophilus PV72/p2, which is involved in the anchoring of the surface-layer protein to the bacterial cell wall layer, is composed of 2-amino-2-deoxy- and 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-mannose, and 2-acetamido-2-deoxy-D-mannuronic acid. The primary structure of the acid-degraded polysaccharide - liberated by HF-treatment from the cell wall - was determined by high-field NMR spectroscopy and mass spectrometry using N-acetylated and hydrolyzed polysaccharide derivatives as well as Smith-degradation. The polysaccharide was shown to consist of a tetrasaccharide repeating unit containing a pyruvic acid acetal at a side-chain 2-acetamido-2-deoxy-*-D-mannopyranosyl residue. Substoichiometric substitutions of the repeating unit were observed concerning the degree of N-acetylation of glucosamine residues and the presence of side-chain linked 2-acetamido-2-deoxy-β-D-glucopyranosyl units.
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.carres.2008.03.029
Created from the Publication Database of the Vienna University of Technology.