Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):
N. Kreuzinger, A.H. Farnleitner, G. Wandl, R. Hornek, R.L. Mach:
"Molecular Biological Methods (DGGE) as an Tool to Investigate Nitrification Inhibition in Wastewater Treatment";
Vortrag: Enviro 2002 Convention & Exhibition and IWA World Water Congress,
Melbourne, Australia (eingeladen);
- 12.04.2002; in: "Proceeding auf CD",
paper no.: e20468a
Incomplete nitrification at an activated sludge plant for biological pre-treatment of rendering plant effluents led to detailed investigations on origin and solution of this problem. Preliminary studies revealed an inhibition of ammonia oxidising microorganisms (AOM) by process waters of the rendering plant to be responsible for the situation. We were able to show a correlation between existence of specific AOM and nitrification capacity. Only Nitrosospira sp. was found in the activated sludge of the rendering plant and another industrial wastewater treatment plant with problems in nitrification, while reference plants without nitrification problems showed Nitrosomonas spp. as predominant ammonia oxidising bacteria. By accompanying engineering investigations and experiments (cross feeding experiments, operation of a two stage laboratory plant) with molecular biological methods (DGGE - Denaturing Gradient Gel Electrophoresis) we were able to elaborate an applicable solution for the rendering plant. Laboratory experiments with a two stage process layout finally provided complete nitrification overcoming the inhibiting nature of process waters from the rendering plant. DGGE analysis of the 2nd stage activated sludge from the laboratory plant showed a shift in population structure from Nitrosospira sp. towards Nitrosomonas spp. simultaneously to the increase of nitrification capacity. Nitrification capacities comparable to full scale municipal wastewater treatment plants could be maintained for more than two month. As the design of wastewater treatment plants for nitrification is linked to the growth characteristics of Nitrosomonas spp., established criteria can be applied for the redesign of the full scale plant.
Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.