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Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):

R. Hornek, A.H. Farnleitner, N. Kreuzinger, R.L. Mach:
"Analysis of ribosomal DNA is sufficient to reveal banding patterns from beta-proteobacterial Ammonia Oxidizers of intact cell in activated sludge";
Vortrag: 10th International Symposium on Microbial Ecology ISME-10 Microbial Planet: Subsurface to Space, Cancun, Mexico (eingeladen); 22.08.2004 - 27.08.2004; in: "Book of Abstracts", (2004), S. 246.



Kurzfassung englisch:
ANALYSIS OF RIBOSOMAL DNA IS SUFFICIENT TO REVEAL BANDING PATTERNS FROM BETA - PROTEOBACTERIAL AMMONIA OXIDIZERS OF INTACT CELLS IN ACTIVATED SLUDGE SAMPLES
R. Hornek 1, A.H. Franleitner 1, N. Kreuzinger 2, R.L. Mach 1
1 Institute for Chemical Engineering, Division of Applied Biochemistry and Gene Technology, Vienna University of Technology, Vienna, Austria
2 Institute for Water Quality and Waste Management, Dep. for Chemistry and Biology of Water, Vienna University of Technology, Vienna, Austria
E-mail: e9204665@student.tuwien.ac.at
Biological nitrogen removal in industrial and municipal wastewater treatment plants (WWTP) is of major importance for maintaining the quality of surface waters. Nitrification capacity depends on the abundance and activity of the respective nitrifying bacterial population and is essential for the construction and upgrading of WWTPs, especially for those treating industrial wastewaters. However, these parameters show significant variation depending on the nature and origin of the treated wastewater. Molecular investigations allow the monitoring of the dynamics of ammonia oxidizing bacteria (AOB) communities which are responsible for ammonia oxidation. The aim of this study was to adapt a rapid nucleic acid extraction technique allowing to simultaneously obtain rRNA, mRNA and DNA from activated sludge. The quality for subsequent molecular analysis was tested with sludge samples of various WWTP differing in wastewater composition and treatment conditions by a comparative screening of the b-AOB community on the 16S rDNA and 16S rRNA level. In addition, the applicability to detect mRNA was evaluated by RT-PCR targeting the gene of the ammonia monooxygenase transcripts. Results indicated that our approach can successfully be applied for studies on the DNA/RNA level in activated sludge systems. Furthermore a remarkable accordance between the corresponding rDNA and rRNA profiles specific for AOB was obtained independent of the investigated treatment plants and conditions. So, this procedure is therefore ideally suited for practical field work in order to support recorded data in sewage treatment plants whenever needed.

Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.