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C. Koch:
"Novel "h-shape" acoustic filter technology applied on yeast cultures";
Vortrag: Seminar Institut für Allgemeine Physik (IAP), TU Wien; 17.03.2009.

When particles in suspensions, such as yeast cells in a culture medium, are subject to ultrasonic standing wave fields, the so-called acoustic radiation forces drive the particles into acoustic pressure nodal planes. This separates the particles from the suspension medium, thus creating a filtering effect.
The recently developed "h-shape filter" is a continous through-put set-up that concentrates suspended particles in one outlet by using the pressure nodal plains as "rails" for the particles, while the cleared medium can be harvested from the other outlet.
This new filtering method is particularly interesting for biotechnology applications. Hence, it is important to assess possible influences on biological cells. In the present study, Saccharomyes cerevisiae (baker's yeast) have been investigated, because they are easy to handle and easily accessible. This talk introduces the h-shape filter and presents experimental results on yeast separation efficiency. Influence is discussed on cell viabilty, cell breakage and the cells' ability to reproduce.