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G. Quintas, J. Kuligowski, B. Lendl:
"On-Line Fourier Transform Infrared Spectrometric Detection in Gradient Capillary Liquid Chromatography Using Nanoliter-Flow Cells";
Analytical Chemistry, 81 (2009), S. 3746 - 3753.

A capillary liquid chromatographic system has been
successfully interfaced with a mid-IR Fourier transform
infrared (FT-IR) spectrometer. Spectra were recorded online
using a micromachined transmission CaF2 cell
(internal volume of 7.5 nL) that was placed on a
dedicated beam condenser attached to the spectrometer.
Linear gradients were run from (50:50) to (35:
65) water (0.05% TFA)/acetonitrile in 15 min for the
separation of standard solutions of four nitrophenols
(4-nitrophenol, 3-methyl-4-nitrophenol, 2,4-dinitrophenol,
and 2-nitrophenol) in a reversed phase system,
providing limits of detection between 35 and 94 ng oncolumn.
The changing background absorption due to
gradient elution was successfully corrected by using a
dedicated algorithm implemented in Matlab. When this
chemometric data treatment was used, highly characteristic
analyte spectra could be recorded as indicated
by correlation coefficients between 89 and 95.8%,
obtained when comparing mid-IR spectra of standard
solutions and the spectra extracted from the chromatogram.