[Zurück]

S. Radel, M. Brandstetter, B. Lendl:
"USW manipulating the location of suspended yeast cells in close proximity to an in-line infrared spectroscopy probe";
Vortrag: USWNet 2009, Stockholm; 30.11.2009 - 01.12.2009; in: "USWNet 2009 Proceedings", (2009).

The growing use of biotechnology as a manufacturing route for e.g. antibiotics and other medical
compounds stimulates the development of reliable sensors for bioprocess purposes. Vibrational
spectroscopy is an optical measurement technique increasingly popular in process analytical chemistry
because of its ability to directly provide molecular specific (bio-)chemical information about a given
sample. The ATR (Attenuated Total Reflection) spectroscopy is a widely used method for mid-infrared
vibrational spectroscopy especially in connection with highly absorbing samples like e.g. aqueous solutions. Only a thin film of some micrometers in the proximity of the ATR sensitive element is
spectroscopically analyzed, beyond this evanescent field the instrument is "blind". This opens the possibility to employ the radiation forces within an USW to manipulate the whereabouts of suspended particles - biological cells in the case of a bioreactor - relative to the ATR. More precisely the field is used to either push the particles towards the optical sensor or away from it. This enables one to detect the infrared spectrum of cells and the supernatant independently.

http://publik.tuwien.ac.at/files/PubDat_181355.pdf