Talks and Poster Presentations (with Proceedings-Entry):
J. Torgersen, A. Baudrimont, N.U Pucher, K. Stadlmann, K. Cicha, C. Heller, R. Liska, J. Stampfl:
"In Vivo Scaffold Fabrication";
Poster: ESAO-ASAIO Winter School 2011,
- 01-29-2011; in: "Advancing Roles: Biomaterials in Artificial Organs and Regenerative Medicine",
D. Falkenhagen, E. F. Leonard, S. Mikhalovsky (ed.);
Two-photon-polymerisation is a rapidly developing technique for the micro- and nanofabrication of three dimensional structures. Its high resolution and the full accessibility of the 3rd order of dimension render it the ideal technique for applications in nanophotonics and biomedicine. Its wide selection of applicable materials makes it especially promising in the field of biomedicine. The 2PP technique permits the reproduction of complex anatomic structures specifically favouring the fabrication of custom made polymeric scaffolds. 2PP structuring does not harm biological tissue since there is a window of transparency for biological material within the used wavelengths. The challenge is to fabricate scaffolds directly in vivo.
This poster reports the fabrication of scaffolds using (meth)acrylate-based photopolymers with embedded living organisms (Caenorhabditis elegans). The structuring was performed with a pulsed laser with a wavelength of 810nm and adjustable power up to 160mW. Using a 20x magnification microscope objective with a numerical aperture of 0.4, a high resolution scaffold with a base area of 300x300Ám and a height of 80Ám could be fabricated. Taking advantage of high laser intensities (writing speed: 300Ám/s) the structuring process took only 12 minutes.
As the stress response of biological material to NIR light is negligible, one can consider environmental stress of chemical origin only. To optimize the conditions we tested the toxicity and reactivity of different resins with a focus on water-based, biocompatible and biodegradable hydrogels together with water soluble, near-infrared initiators suitable for 2PP.
scaffold, Two-photon-polymerisation, high resolution, complex anatomic structures, in vivo, photopolymer, fs-pulsed laser
Electronic version of the publication:
Created from the Publication Database of the Vienna University of Technology.