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Zeitschriftenartikel:

C. Carrillo-Carrión, S. Armenta Estrela, B. Simonet, M. Valcárcel, B. Lendl:
"Determination of Pyrimidine and Purine Bases by Reversed-Phase Capillary Liquid Chromatography with At-Line Surface-Enhanced Raman Spectroscopic Detection Employing a Novel SERS Substrate Based on ZnS/CdSe Silver-Quantum Dots";
Analytical Chemistry, 83 (2011), 24; S. 9391 - 9398.



Kurzfassung deutsch:
We have developed a new SERS substrate based
on the reduction of silver nitrate in the presence of ZnS-capped
CdSe quantum dots. This substrate showed higher sensitivities
as compared to a hydroxylamine-reduced silver sol. On the basis
of this new substrate, at-line SERS detection was coupled with
capillary liquid chromatography (cap-LC) for the separation
and selective determination of pyrimidine and purine bases. For
this purpose, wells of a dedicated microtiter plate were loaded
with 20 μL of the SERS substrate and placed on an automated
x,y translation stage. A flow-through microdispenser capable of ejecting 50 pL droplets, at a frequency 100 Hz, was used as the
interface to connect the cap-LC system to the wells loaded with SERS substrate. A detailed study of the dependence of both the
separation and the surface-enhanced Raman spectra of each base on the pH was performed to optimize the system for maximum
sensitivity and selectivity. Highly satisfactory analytical figures of merit were obtained for the six investigated bases (cytosine,
xanthine, hypoxanthine, guanine, thymine, and adenine) with detection limits ranging between 0.2 and 0.3 ng injected on the
capillary LC column, and the precisions were in the range of 3.0 6.3%.

Kurzfassung englisch:
We have developed a new SERS substrate based
on the reduction of silver nitrate in the presence of ZnS-capped
CdSe quantum dots. This substrate showed higher sensitivities
as compared to a hydroxylamine-reduced silver sol. On the basis
of this new substrate, at-line SERS detection was coupled with
capillary liquid chromatography (cap-LC) for the separation
and selective determination of pyrimidine and purine bases. For
this purpose, wells of a dedicated microtiter plate were loaded
with 20 μL of the SERS substrate and placed on an automated
x,y translation stage. A flow-through microdispenser capable of ejecting 50 pL droplets, at a frequency 100 Hz, was used as the
interface to connect the cap-LC system to the wells loaded with SERS substrate. A detailed study of the dependence of both the
separation and the surface-enhanced Raman spectra of each base on the pH was performed to optimize the system for maximum
sensitivity and selectivity. Highly satisfactory analytical figures of merit were obtained for the six investigated bases (cytosine,
xanthine, hypoxanthine, guanine, thymine, and adenine) with detection limits ranging between 0.2 and 0.3 ng injected on the
capillary LC column, and the precisions were in the range of 3.0 6.3%.


"Offizielle" elektronische Version der Publikation (entsprechend ihrem Digital Object Identifier - DOI)
http://dx.doi.org/10.1021/ac201821q


Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.