Talks and Poster Presentations (with Proceedings-Entry):
A. Ovsianikov, S. Nürnberger, J. Torgersen, Z. Li, H. Redl, R. Liska, J. Stampfl:
"Fabrication of elastic 3D scaffolds by two-photon polymerization";
Talk: Materials Science and Engineering 2012,
- 09-27-2012; in: "MSE Programme",
Two-photon polymerization (2PP) is a method based on localized cross-linking of photopolymers, induced by femtosecond laser pulses. Its capability of creating complex 3D structures with high precision and reproducibility is particularly appealing for the field of regenerative medicine and tissue engineering for the fabrication of scaffolds . The primary function of the scaffold structure is to provide a micro- and nano-structured 3D environment for the cells to migrate and to proliferate in . The specific properties of the scaffold have to resemble natural cell environment, providing appropriate geometry and instructive cues needed to maintain cell phenotype and behavior . Once the cells of the engineered tissue have built their own connective matrix, the scaffold becomes redundant. Therefore, materials that are biodegradable on a similar timescale as the production of the extra-cellular matrix of the engineered tissue are preferred. In comparison to other technologies currently applied for the fabrication of scaffolds, the advantage of 2PP technique is a combination of unprecedented resolution, high reproducibility, and the ability to generate true 3D structures directly from CAD input .
In this contribution our recent results on fabrication of scaffolds by 2PP of novel biodegradable urethane-based photoelastomer are presented . The new material supports submicron spatial resolution of the 2PP. The high reactivity of the developed photoelastomer allows 2PP at the scanning speed of over 10 mm/s, facilitating rapid fabrication of relatively large structures. Our results show that stable scaffolds with porosities of over 70% can be produced. Such important issues as biocompatibility and structureability of applied material will be discussed. Preliminary studies indicate that these materials support adhesion and proliferation of different cell types. Our results emphasize the potential of the 2PP method for realization of rationally engineered 3D scaffolds. The presentation is supported by numerous examples.
Electronic version of the publication:
Created from the Publication Database of the Vienna University of Technology.