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Zeitschriftenartikel:

A. Alexander, D. Biedermann, M. Fink, M.D. Mihovilovic, T. Mattes:
"Enantioselective Oxidation by a Cyclohexanone Monooxygenase from the Xenobiotic-Degrading Polaromonas sp. strain JS666";
Journal of Molecular Catalysis B Enzymatic, 78 (2012), S. 105 - 110.



Kurzfassung englisch:
A cyclohexanone monooxygenase (CHMO) from the xenobiotic-degrading Polaromonas sp. strain JS666 was heterologously expressed in Escherichia coli, and its ability to catalyze enantio- and regiodivergent oxidations of prochiral and racemic ketones was investigated. The expression system was also used to evaluate this enzyme's potential role in the oxidation of cis-1,2-dichloroethene (cDCE), a groundwater pollutant for which strain JS666 is the only known assimilator. The substrate enantiopreference and -selectivity of the strain JS666 CHMO is similar to that of other CHMO-type enzymes: of note is this enzyme's excellent stereodiscrimination of 2-substituted cyclic ketones. The expression system exhibits no activity with ethene or cDCE as substrates under the tested conditions. Phylogenetic analysis shows that sequence variability among cyclohexanone monooxygenases could be a rich source of new enzyme activities and attributes. (C) 2012 Elsevier B.V. All rights reserved.

Schlagworte:
Cyclohexanone monooxygenase; Enantioselective oxidation; Baeyer-Villiger oxidation; Chiral lactone; Cis-1,2-dichloroethene


"Offizielle" elektronische Version der Publikation (entsprechend ihrem Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.molcatb.2012.03.002


Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.