Publications in Scientific Journals:
A. Alexander, D. Biedermann, M. Fink, M.D. Mihovilovic, T. Mattes:
"Enantioselective Oxidation by a Cyclohexanone Monooxygenase from the Xenobiotic-Degrading Polaromonas sp. strain JS666";
Journal of Molecular Catalysis B Enzymatic,
A cyclohexanone monooxygenase (CHMO) from the xenobiotic-degrading Polaromonas sp. strain JS666 was heterologously expressed in Escherichia coli, and its ability to catalyze enantio- and regiodivergent oxidations of prochiral and racemic ketones was investigated. The expression system was also used to evaluate this enzyme's potential role in the oxidation of cis-1,2-dichloroethene (cDCE), a groundwater pollutant for which strain JS666 is the only known assimilator. The substrate enantiopreference and -selectivity of the strain JS666 CHMO is similar to that of other CHMO-type enzymes: of note is this enzyme's excellent stereodiscrimination of 2-substituted cyclic ketones. The expression system exhibits no activity with ethene or cDCE as substrates under the tested conditions. Phylogenetic analysis shows that sequence variability among cyclohexanone monooxygenases could be a rich source of new enzyme activities and attributes. (C) 2012 Elsevier B.V. All rights reserved.
Cyclohexanone monooxygenase; Enantioselective oxidation; Baeyer-Villiger oxidation; Chiral lactone; Cis-1,2-dichloroethene
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
Created from the Publication Database of the Vienna University of Technology.