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Publications in Scientific Journals:

S. Mathur, A. Erdem, C. Cavelius, S. Barth, J. Altmayer:
"Amplified Electrochemical DNA-Sensing of Nanostructured Metal Oxide Films deposited in Disposible Graphite Electrodes Functionalized by Chemical Vapor Deposition";
Sensors and Actuators B: Chemical, 136 (2009), 432 - 437.



English abstract:
Metal oxide nanostructures offer interesting possibilities to design functional surfaces for biosensing applications, for instance, through higher surface area leading to enhanced immobilization of biomolecules, which increases the detection limit. Herein, an amplified electrochemical sensing method has been presented for the detection of DNA based on the readout resulting from chemical oxidation of guanine on nanoscaled metal oxides (TiO2, SnO2 and Fe3O4) obtained by chemical vapor deposition (CVD) onto pencil graphite electrode (PGE) as electrochemical transducer. The proposed strategy is suitable to produce cost-effective disposable sensor elements enabling quantitative detection of nanomolar concentrations of DNA. When preparing these metal oxide surfaces by CVD onto PGEs, the various experimental conditions; such as, the effect of different concentrations of 20 mer-bases DNA oligonucleotide (ODN20), and the surface pretreatment steps were Studied to obtain better surface properties for DNA immobilization. The detection limit estimated for signal-to-noise ratios >3 corresponds to 21.3, 519 and 45.8 nmole/ml ODN20 concentrations for PGEs modified with TiO2, SnO2 and Fe3O4 films, respectively. The electrochemical detection of DNA onto metal oxide@PGEs is discussed together with the application potential.

Keywords:
DNA; Nanostructured metal oxides; Chemical vapor deposition (CVD); Metal oxide-modified electrodes; Pencil graphite electrodes (PGEs); Guanine oxidation signal


"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.snb.2008.11.049


Created from the Publication Database of the Vienna University of Technology.