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E. Klotzsch, G. Schütz:
"A critical survey of methods to detect plasma membrane rafts";
Philosophical Transactions of the Royal Society B: Biological Sciences, 368 (2013), 201200331 - 2012003317.

The plasma membrane is still one of the enigmatic cellular structures.
Although the microscopic structure is getting clearer, not much is known
about the organization at the nanometre level. Experimental difficulties
have precluded unambiguous approaches, making the current picture
rather fuzzy. In consequence, a variety of different membrane models has
been proposed over the years, on the basis of different experimental
strategies. Recent data obtained via high-resolution single-molecule
microscopy shed new light on the existing hypotheses. We thus think it is
a good time for reviewing the consistency of the existing models with the
new data. In this paper, we summarize the available models in ten propositions,
each of which is discussed critically with respect to the applied
technologies and the strengths and weaknesses of the approaches. Our
aim is to provide the reader with a sound basis for his own assessment.
We close this chapter by exposing our picture of the membrane organization
at the nanoscale.

singe molecule microscopy, FRET, single particle tracking, membrane rafts, plasma membrane, lipids