Publications in Scientific Journals:

R.M. Werkmeister, A. Alex, S. Kaya, A. Unterhuber, B. Hofer, J. Riedl, M. Bronhagl, M. Vietauer, D. Schmidl, T. Schmoll, G. Garhöfer, M. Drexler, R. Leitgeb, M. Gröschl, L. Schmetterer:
"Measurement of tear film thickness using ultrahigh-resolution Optical Coherence Tomography";
Investigative Ophthalmology & Visual Science, 54 (2013), 5578 - 5583.

English abstract:
PURPOSE. To visualize the precorneal tear film with ultrahigh-resolution spectral domain
optical coherence tomography, enabling quantification of tear film thickness in healthy
METHODS. A custom-built spectral domain optical coherence tomography system
comprising a broadband titanium:sapphire laser operating at 800 nm and a high-speed
charge coupled device (CCD) camera with a read-out rate of 47 kHz was used for
measurement of precorneal tear film thickness. The system provides a theoretical axial
resolution of 1.2 lm in tissue. The signal-to-noise ratio close to the zero delay was
measured with 94 dB. A total of 26 healthy subjects were included in this study.
Measurement was started immediately after blinking and averaged over a period of 1
second. In a subset of eight healthy subjects, the reproducibility of the approach was
studied by measuring the tear film thickness every 10 minutes over 1 hour.
RESULTS. The average central tear film thickness of the measured population was 4.79 6 0.88
lm. Reproducibility was very high, with an intraclass correlation coefficient of 0.97. A
breakup of the tear film was observed in one subject after 14 seconds.
CONCLUSIONS. Our data indicate that the human precorneal tear film can be measured with
excellent reproducibility using ultrahigh-resolution optical coherence tomography. This
technique may be a valuable tool in the management of dry eye syndrome. (ClinicalTrials.gov
number, NCT01746602.)

optical coherence tomography, tear film thickness, dry eyes

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