Publications in Scientific Journals:
F. Leipold, F. Rudroff, M.D. Mihovilovic, U. Bornscheuer:
"The Steroid Monooxygenase from Rhodococcus rhodochrous - a Versatile Biocatalyst";
Tetrahedron - Asymmetry,
The substrate scope of a steroid monooxygenase (STMO) from Rhodococcus rhodochrous DSM 43269 was
investigated for a large range of different ketone substrates. These studies revealed that this enzyme not
only oxygenates steroids, but also ketone moieties of a series of other open-chain ketones, such as cyclohexyl
methyl ketone, cyclopentyl methyl ketone, and 3-acetylindole. Furthermore, the STMO catalyzed
the oxygenation of cyclobutanone derivatives. Comparative biotransformations with recombinant Escherichia
coli resting cells harboring the STMO, the cycloalkanone monooxygenase (CAMO) from Cylindrocarpon
radicicola or the cyclohexanone monooxygenase (CHMO) from Acinetobacter calcoaceticus revealed
that the STMO is enantiodivergent compared to the CHMO-type. Moreover, the STMO resulted in a higher
enantiomeric excess of the product lactones compared to the known BVMOs of the same enantiopreference,
such as cyclopentanone monooxygenases.
Created from the Publication Database of the Vienna University of Technology.