Talks and Poster Presentations (with Proceedings-Entry):
A. Ovsianikov, K. Haslinger, X.H. Qin, Z. Li, P. Gruber, J. Torgersen, S. Van Vlierberghe, P. Dubruel, R. Liska, J. Stampfl:
"Laser microfabrication of 3D CAD structures from photosensitive hydrogel precursors";
Talk: International Conference on Materials for Advanced Technologies,
- 07-05-2013; in: "ICMAT 2013 - 7th International Conference on Materials for Advanced Technologies",
Hydrogels are widely used as 3D matrices for cell growth owing to similarity of their mechanical and diffusivity properties to the natural cell environment. A number of synthetic and natural hydrogels has been developed and used as 3D scaffold for studies of cell behavior and tissue formation.
Photoinduced polymerization is one of the most popular approaches, since it provided temporal and spatial control over the hydrogel formation process. Nevertheless, conventional mask-lithography-like methods are limited to fabrication of semi-2D patterns. In order to produce 3D constructs additional handling or assembly steps are required.
Here we present a method capable of producing 3D hydrogel structures with high spatial resolution directly from computer aided design (CAD) model. Cross-linking of photosensitive hydrogel precursors is achieved by two-photon polymerization (2PP) - a technique based on localized absorption of femtosecond pulses within the focus of the laser beam. Specially developed photoinitiators allow efficient 2PP-processing of material formulations with over 80 % initial water content at a scanning speed of up to 5 m/s.
In this contribution, our recent results on 2PP-fabrication of hydrogel structures from formulations based on natural (gelatin, hyaluronic acid, bovine serum albumin), as well as synthetic (PEG) polymers are presented. Developed two-photon-active photoinitiators are compared with regard to their performance for 2PP of water-based photopolymers. The presentation is supported by numerous examples.
Created from the Publication Database of the Vienna University of Technology.