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Zeitschriftenartikel:

T. Beskers, M. Brandstetter, J. Kuligowski, G. Quintas, M. Wilhelm, B. Lendl:
"High performance liquid chromatography with mid-infrared detection based on a broadly tunable quantum cascade laser";
Analyst, 139 (2014), S. 2057 - 2064.



Kurzfassung englisch:
This work introduces a tunable mid-infrared (mid-IR) external cavity quantum cascade laser (EC-QCL) as a
new molecular specific detector in liquid chromatography. An EC-QCL with a maximum tunability of
200 cm 1 (1030-1230 cm 1) was coupled to isocratic high performance liquid chromatography (HPLC)
for the separation of sugars with a cation exchange column (counter ion: Ca2+) and distilled water as the
mobile phase. Transmission measurements in a 165 mm thick flow cell allowed for on-line coupling and
independent quantification of glucose, fructose and sucrose in the concentration range from 5 mg mL 1
to 100 mg mL 1 in several beverages. The results obtained with the EC-QCL detector were found to be
in good agreement with those obtained using a differential refractive index detector as a reference. The
standard deviation of the method for the linear calibration was better than 5 mg mL 1 for all sugars and
reached a minimum of 1.9 mg mL 1, while the DRI detector reached a minimum of 1 mg mL 1. Besides
the quantification of sugars for which a calibration was performed, also chromatographic peaks of other
components could be identified on the basis of their IR absorption spectra. This includes taurine,
ethanol, and sorbitol.


"Offizielle" elektronische Version der Publikation (entsprechend ihrem Digital Object Identifier - DOI)
http://dx.doi.org/10.1039/c3an01546g


Erstellt aus der Publikationsdatenbank der Technischen Universitšt Wien.