Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):
W. Nischkauer, F. Vanhaecke, C. Herwig, A. Limbeck:
"Quantification of micro nutrients in fermentation media using LA-ICP-OES/MS analysis of dried sample droplets";
Vortrag: European Symposium on Atomic Spectrometry 2014,
Prag, Czech Republic;
16.03.2014
- 21.03.2014; in: "Book of Abstracts",
(2014),
ISBN: 978-80-905704-1-2;
104 S.
Kurzfassung englisch:
Controlling nutrients in fermentation media has become a key aspect in process monitoring as biochemical processes gain more and more importance in industry and research. Especially micro nutrients ("essential elements") are difficult to quantify. Firstly, they are present in low concentrations only, and secondly, they require element-specific detection. Therefore, essential elements are usually quantified offline with suitable atomic spectroscopy techniques such as inductively coupled plasma optical emission spectroscopy (ICP-OES) or mass spectrometry (ICP-MS) which offer detection limits in the range of ng mL-1 and pg mL-1, respectively.
ICP-OES and ICP-MS are rugged techniques but fermentation media are nevertheless challenging samples. They contain large concentrations of organic compounds as well as inorganic salts, making it necessary to dilute the samples. Otherwise, nebulizers and cones might be blocked. Diluting the samples is usually done off-site and comes at the cost of reduced detection power. This calls for alternatives in sample preparation to allow on-site sample preparation and to provide reasonable sensitivity.
One possible way to meet these conditions is by preparing dried droplets of the samples. To give an example, it is common practice in clinical research to stabilize and store blood or urine on paper cards. Elemental analysis of such samples can then be performed later on, using suitable solid-sampling techniques.
We present an approach for stabilizing fermentation media on filter paper disks for the subsequent quantification with laser ablation ICP-OES and ICP-MS using external matrix-adjusted calibration. As dilution is avoided with our approach, method quantification limits are comparable with conventional measurement approaches. The accuracy of our proposed method is shown by standard addition and by comparison with conventional approaches.
Schlagworte:
LA-ICP-OES, dried-droplet quantification, Biochemical fermentation
Zugeordnete Projekte:
Projektleitung Andreas Limbeck:
Nanoparticles for improved trace metal analysis
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.