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Vorträge und Posterpräsentationen (mit Tagungsband-Eintrag):

M. Markovic, J. Van Hoorick, S. Van Vlierberghe, T. Fowler, O. Hoffmann, P. Dubruel, A. Ovsianikov:
"Seeding 3D CAD scaffolds with photopolymerizable cellhydrogel suspension";
Vortrag: 2014 Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Meeting (TERMIS-AP 2014), Daegu, S. Korea; 24.09.2014 - 27.09.2014; in: "2014 Tissue Engineering and Regenerative Medicine International Society-Asia Pacific Meeting (TERMIS-AP 2014)", (2014), S. #.



Kurzfassung englisch:
Hydrogels are widely used as 3D matrices for cell growth owing to similarity of their mechanical and
diffusivity properties to the natural extracellular matrix (ECM). Furthermore, encapsulation of living cells
within the hydrogel allows to produce constructs with high initial cell loading and intimate cell-matrix
contact, similar to that of the natural ECM. In this contribution the results of the seeding of macroporous
poly-lactic acid (PLA) based scaffolds with the help of photopolymerizable gelatin as a cell delivery
material are presented. The 3D scaffolds were produced from PLA in accordance to computer aided
design (CAD) model by means of fused deposition modeling (FDM, Ultimaker). A solution of
methacrylamide-modified gelatin (gel-MOD) in cell culture medium was used as a cell carrier material
for seeding. A 107/ml suspension of MC3T3 cells in gel-MOD was introduced into the pores of the
scaffold and then photopolymerized. Our results indicate that such seeding procedure facilitates
delivering cells into the pores of the scaffolds at high density and homogeneous distribution. The
proliferation of the cell within the scaffolds was monitored using a Presto Blue assay for the course of
three weeks. The data was verified by DNA quantification at different time points. An upregulation of the
osteocalcin expression and a downregulation of RUNX2 indicate the osteogenic differentiation of
MC3T3s. These results are confirmed by the positive alkaline phosphatase (ALP) staining. Our findings
show that photoinduced encapsulation of cells within a cell-interactive hydrogel is a valuable route to
introduce cells throughout 3D scaffolds for bone tissue engineering.

Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.