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H. W. Yoo, M. E. van Royen, W. A. van Cappellen, A. B. Houtsmuller, M. Verhaegen, G. Schitter:
"Automated Spherical Aberration Correction in Scanning Confocal Microscopy";
Review of Scientific Instruments, 85 (2014), 123706; S. 1 - 9.

Mismatch between the refractive indexes of immersion media and glass coverslips introduces spherical
aberrations in microscopes especially for high numerical aperture objectives. This contribution
demonstrates an automated adjustment of the coverslip correction collar in scanning confocal microscopy
to compensate for spherical aberrations due to coverslip thickness mismatch. With a motorized
coverslip correction collar, the adjustment procedure consists of xz image scans, image processing,
correction quality evaluation, the mismatch estimation, and eventually the optimal adjustment
of the correction collar. For fast correction with less photodamage, coarse-fine Gaussian fitting algorithms
are proposed and evaluated with various specimen for their estimation accuracy. The benefits
of the proposed automated correction are demonstrated for various coverslips with biological specimens,
showing the optimized resolution of the confocal microscope.

Coverslip correction collar, spherical aberration, confocal laser scanning microscope, aberartion correction

http://dx.doi.org/10.1063/1.4904370