Publications in Scientific Journals:
N. Oberleitner, C. Peters, F. Rudroff, U. Bornscheuer, M.D. Mihovilovic:
"In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, an enoate reductases and a Baeyer-Villiger monooxygenase";
Journal of Biotechnology,
192
(2014),
393
- 399.
English abstract:
An artificial enzyme cascade composed of an alc. dehydrogenase, an enoate reductase and a Baeyer-Villiger monooxygenase was investigated in vitro to gain deeper mechanistic insights and understand the assets and drawbacks of this multi-step biocatalysis. Several substrates composed of different structural motifs were examd. and provided access to functionalized chiral compds. in high yields (up to >99%) and optical purities (up to >99%). Hence, the applicability of the presented enzymic cascade was exploited for the synthesis of biorenewable polyesters.
Keywords:
Multi-step biocatalysis, Baeyer-Villiger monooxygenase, Biorenewable polyesters, Enoate reductase, Alcohol dehydrogenase
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.jbiotec.2014.04.0
Electronic version of the publication:
http://publik.tuwien.ac.at/files/PubDat_236623.pdf
Created from the Publication Database of the Vienna University of Technology.