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Publications in Scientific Journals:

N. Oberleitner, C. Peters, F. Rudroff, U. Bornscheuer, M.D. Mihovilovic:
"In vitro characterization of an enzymatic redox cascade composed of an alcohol dehydrogenase, an enoate reductases and a Baeyer-​Villiger monooxygenase";
Journal of Biotechnology, 192 (2014), 393 - 399.



English abstract:
An artificial enzyme cascade composed of an alc. dehydrogenase, an enoate reductase and a Baeyer-​Villiger monooxygenase was investigated in vitro to gain deeper mechanistic insights and understand the assets and drawbacks of this multi-​step biocatalysis. Several substrates composed of different structural motifs were examd. and provided access to functionalized chiral compds. in high yields (up to >99​%) and optical purities (up to >99​%)​. Hence, the applicability of the presented enzymic cascade was exploited for the synthesis of biorenewable polyesters.

Keywords:
Multi-step biocatalysis, Baeyer-Villiger monooxygenase, Biorenewable polyesters, Enoate reductase, Alcohol dehydrogenase


"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.jbiotec.2014.04.0

Electronic version of the publication:
http://publik.tuwien.ac.at/files/PubDat_236623.pdf


Created from the Publication Database of the Vienna University of Technology.