Zeitschriftenartikel:
C. Peters, R. Koelzsch, M. Kadow, L. Skalden, F. Rudroff, M.D. Mihovilovic, U. Bornscheuer:
"Identification, Characterization, and Application of Three Enoate Reductases from Pseudomonas putida in In Vitro Enzyme Cascade Reactions";
ChemCatChem,
6
(2014),
S. 1021
- 1027.
Kurzfassung englisch:
Enoate reductases are versatile enzymes for the enantio- and regioselective addn. of hydrogen to double bonds. We identified three EREDs (XenA, XenB, NemA) from Pseudomonas putida ATCC 17453 through a sequence motif search. In addn. to cloning, functional expression, and biochem. characterization of these enzymes, the enoate reductases were also applied in enzyme cascade reactions in combination with a Baeyer-Villiger monooxygenase and an alc. dehydrogenase to produce lactones.
Schlagworte:
biocatalysis · enantioselectivity · enoate reductase · enzymes · protein expression
"Offizielle" elektronische Version der Publikation (entsprechend ihrem Digital Object Identifier - DOI)
http://dx.doi.org/10.1002/cctc.201300957
Elektronische Version der Publikation:
http://publik.tuwien.ac.at/files/PubDat_236639.pdf
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.