Publications in Scientific Journals:
C. Peters, R. Koelzsch, M. Kadow, L. Skalden, F. Rudroff, M.D. Mihovilovic, U. Bornscheuer:
"Identification, Characterization, and Application of Three Enoate Reductases from Pseudomonas putida in In Vitro Enzyme Cascade Reactions";
Enoate reductases are versatile enzymes for the enantio- and regioselective addn. of hydrogen to double bonds. We identified three EREDs (XenA, XenB, NemA) from Pseudomonas putida ATCC 17453 through a sequence motif search. In addn. to cloning, functional expression, and biochem. characterization of these enzymes, the enoate reductases were also applied in enzyme cascade reactions in combination with a Baeyer-Villiger monooxygenase and an alc. dehydrogenase to produce lactones.
biocatalysis · enantioselectivity · enoate reductase · enzymes · protein expression
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
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Created from the Publication Database of the Vienna University of Technology.