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Publications in Scientific Journals:

C. Peters, R. Koelzsch, M. Kadow, L. Skalden, F. Rudroff, M.D. Mihovilovic, U. Bornscheuer:
"Identification, Characterization, and Application of Three Enoate Reductases from Pseudomonas putida in In Vitro Enzyme Cascade Reactions";
ChemCatChem, 6 (2014), 1021 - 1027.



English abstract:
Enoate reductases are versatile enzymes for the enantio- and regioselective addn. of hydrogen to double bonds. We identified three EREDs (XenA, XenB, NemA) from Pseudomonas putida ATCC 17453 through a sequence motif search. In addn. to cloning, functional expression, and biochem. characterization of these enzymes, the enoate reductases were also applied in enzyme cascade reactions in combination with a Baeyer-​Villiger monooxygenase and an alc. dehydrogenase to produce lactones.

Keywords:
biocatalysis enantioselectivity enoate reductase enzymes protein expression


"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1002/cctc.201300957

Electronic version of the publication:
http://publik.tuwien.ac.at/files/PubDat_236639.pdf


Created from the Publication Database of the Vienna University of Technology.