[Zurück]

A. Balbekova, G. Ramer, B. Lendl:
"Monitoring of dynamic changes in poly-L-lysine thin films by time resolved photothermal infrared nanoscopy";
Poster: IRDG Christmas Meeting 2014, London, UK; 18.12.2014.

Over the past few years photothermal infrared (IR) nanoscopy attracted great scientific interest due to the possibility of nondestructive and label free analysis of biological samples at the nanoscale. [1] The working principle of this technique is based on the detection of the local thermal expansion of a sample induced by absorption of pulsed IR radiation. By tuning the laser at different wavelengths and measuring the cantilever deflection the absorption spectrum of the sample is determined.
Recently, important advances in photothermal IR nanoscopy with regard to high spatial resolution (better than 25 nm) and monolayer sensitivity were developed. [2] However, so far, only static systems have been investigated. In the current work a dynamic system using photothermal IR nanoscopy was investigated for the first time. Investigations of conformational transitions from β-sheet to α-helix in poly‑L‑lysine (PLL) thin film initiated by change in relative humidity are presented.

photothermal infrared nanoscopy, poly-L-lysine thin film, AFM, infrared spectroscopy

http://publik.tuwien.ac.at/files/PubDat_237205.pdf