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F. Baumgart, G. Schütz:
"Detecting protein association at the T cell plasma membrane";
Biochimica et Biophysica Acta, 1853 (2015), 791 - 801.

At themoment,many models on T cell signaling rely on results obtained via rather indirect methodologies,which
makes direct comparison and conclusions to the in vivo situation difficult. Recently, a variety of new imaging
methods were developed, which have the potential to directly shed light onto the mysteries of protein association
at the T cell membrane. While the new modalities are extremely promising, for a broad readership it may
be difficult to judge the results, since technological shortcomings are not always obvious. In this review article,
we put key questions on the mechanism of protein interactions in the T cell plasma membrane into relation
with techniques that allow to address such questions. We discuss applicability of the techniques, their strengths
and weaknesses. This article is part of a Special Issue entitled: Nanoscale membrane organisation and signalling.

Protein interaction T cell plasma membrane Single molecule microscopy