Vorträge und Posterpräsentationen (ohne Tagungsband-Eintrag):
A. Schwaighofer, M. Alcaraz, C. Kristament, G. Ramer, M. Brandstetter, H. Goicoechea, B. Lendl:
"EC-QC laser spectroscopy for mid-IR transmission measurements of proteins in aqueous solution";
Vortrag: ITQW - The 13th International Conference on Intersubband Transitions in Quantum Wells,
Wien;
06.09.2015
- 11.09.2015.
Kurzfassung englisch:
We introduce a rugged setup for mid-IR transmission measurements of the protein amide I band in aqueous solution at large optical paths using an EC-QCL operated in pulsed mode combined. An advanced data processing protocol was devised to overcome non-random fluctuations in single beam spectra inherent to EC-QCL emission curves. To provide for temperature stability, a custom-made temperature-controlled flow cell was designed. A method for frequency calibration of EC-QCLs has been elaborated to reference the acquired IR spectra to the absolute positions of the water vapor absorption bands.
Employing this setup, characteristic spectral features of proteins with different secondary structures have been elucidated at concentrations as low as 2.5 mg mL-1.
Schlagworte:
EC-QCL, infrared, protein, secondary structure, laser
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.