Vorträge und Posterpräsentationen (ohne Tagungsband-Eintrag):
A. Schwaighofer, M. Alcaraz, C. Kristament, H. Goicoechea, B. Lendl:
"EC-QC laser mid-IR transmission spectroscopy for monitoring beta-aggregation in alcohol-denaturated proteins";
Poster: ICAVS 8,
Wien;
12.07.2015
- 17.07.2015.
Kurzfassung englisch:
A rugged setup for mid-IR transmission measurements at large optical paths using an EC-QCL was employed for monitoring beta-aggregation in alcohol-denaturated proteins. Exposure of beta-rich alpha-chymotrypsin to 2,2,2-trifluoroethanol leads to immediate formation of nonnative alpha-helices. The subsequent gradual transition to intermolecular beta-sheet aggregates was monitored by mid-IR laser transmission spectroscopy. Multivariate curve resolution (MCR) was employed for analysis of spectral and concentration profiles of the temporal transition between alpha-helices an intermolecular beta-sheets
Schlagworte:
mid-IR spectroscopy, EC-QCL, protein structure, denaturation, -aggregation, multivariate curve resolution
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.