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Talks and Poster Presentations (with Proceedings-Entry):

M. Bonta, B. Hegedus, A. Limbeck:
"Trace element analysis and quantification in tumor tissues using LA-ICP- MS";
Poster: Metallomics 2015, Beijing, China; 09-09-2015 - 09-12-2015; in: "Book of Abstracts", (2015), 1 pages.



English abstract:
Platinum-containing drugs such as cisplatin, carboplatin and others have been widely used for anti-cancer treatment for more than four decades. However, though extensively studied, especially resistance mechanisms of certain tumor types still have not been fully revealed. Detailed investigations on drug incorporation and its conversion in cellular systems can help to gain knowledge about uptake pathways and may lead to the development of improved treatment strategies. Due to its high sensitivity and the possibility of laterally resolved analysis, laser ablationinductively coupled plasma-mass spectrometry (LA-ICP-MS) is perfectly suitable for the analysis of platinum and other trace elements in cell cultures, xenografts, as well as tissue samples from animal models and/or human patients. Combined with traditional histological and immunohistochemical techniques, and other mass spectrometric imaging techniques (e.g., ToF-SIMS, MALDI), an in-depth analysis of biological processes beyond the state-of-the-art-knowledge is possible. This work has been focused on the analysis of human malignant mesothelioma samples, where cytostatic agents containing platinum are commonly used as an anti-tumor treatment. Besides platinum, also investigations on other physiologically relevant trace metals have been performed. Recent developments in trace element imaging using LA-ICP-MS including novel multi-elemental quantification approaches, improvements regarding lateral resolution, as well as medical application examples will be presented.

Keywords:
LA-ICP-MS, platinum containing drugs, imaging, tissue analysis

Created from the Publication Database of the Vienna University of Technology.