Vorträge und Posterpräsentationen (ohne Tagungsband-Eintrag):
B. Lendl, A. Schwaighofer, M. Alcaraz:
"EC-QC laser spectroscopy for mid-IR transmission measurements of proteins in aqueous solutions";
Vortrag: SCIX 2015,
Providence, RI, USA (eingeladen);
27.09.2015
- 02.10.2015.
Kurzfassung englisch:
Abstract:Mid-IR transmission measurements of the protein amide I band in aqueous solution at large optical paths is presented. A room-temperature operated tunable external-cavity quantum cascade laser (EC-QCL) operated in pulsed mode allowed applying path length of up to 38 µm. To minimize temperature-induced variations caused by background absorption of the bending vibration of water overlapping with the amide I region, a highly-stable temperature control unit with relative temperature stability within 0.005 °C was developed. An advanced data processing protocol (COW) was established to overcome fluctuations in the fine structure of the emission curve that are inherent to the employed EC-QCL due to its mechanical instabilities. Employing this setup, characteristic spectral features of five well-studied proteins exhibiting different secondary structures could be elucidated at concentrations as low as 2.5 mg ml-1. In addition recent results on protein folding studied by EC-QCL spectroscopy will be presented, too.
Erstellt aus der Publikationsdatenbank der Technischen Universität Wien.