[Zurück]

C. Kristament, M. Brandstetter, A. Schwaighofer, M. Alcaraz, B. Lendl:
"Mid-IR Quantum Cascade Lasers as an Enabling Technology for Analytical Chemistry";
in: "CLEO: Science and Innovations, CLEO-SI 2015", Optical Society of America (OSA), 2015, ISBN: 978-155752968-8, 2 S.

The qualitative analysis of mid-IR spectra of aqueous protein solutions provides information on the secondary structure elements present in the proteins under study. In this work an external cavity (EC)-QCL was used for performing transmission measurements of different aqueous protein solutions in a CaF2 flow cell with optical paths up to 75 µm. The strong temperature dependence of the 2 band of water overlapping the amide I absorption band
(~1640 cm-1) required a temperature stability of +/- 0.005°C. Different proteins solutions could be successfully analyzed for their dominating secondary structure in the low g/L concentration range.

http://dx.doi.org/10.1364/CLEO_SI.2015.SM1O.3