Talks and Poster Presentations (with Proceedings-Entry):
E. Zerobin, S. Baudis, Z. Tomasikova, X.H. Qin, M. Markovic, P. Gruber, J. Stampfl, A. Ovsianikov, R. Liska:
"Micro fabrication of hydrogels based on hyaluronic acid";
Talk: 6th Bratislava Young Polymer Scientists Workshop,
High Tatras, Slovakia;
- 03-18-2016; in: "BYPoS Workshop book",
book of abstracts,
Hyaluronic acid (HA) is a glycosaminoglycane which is widespread in the connective tissue of the human body such as skin, cartilage and in the vitreous body of the eye. Furthermore, it is an FDA approved, water-soluble polymer that has already been extensively used in cosmetics and medicine because HA hydrogels are permeable for nutrients and oxygen.
Biocompatible hydrogels can be obtained by covalent crosslinking of modified HA. For this purpose, double bonds are introduced onto the HA backbone and liquid hydrogel precursor formulations are then solidified by exposure to light using the concept of photo-polymerization. This concept enables light-based 3D printing.
In this work, HA was modified with vinylester groups by an enzymatic transesterification reaction resulting in HA-vinylesters (HAVE)1. Since the reactivity of vinylesters can be increased by thiol-ene chemistry2, dithiols were used for the crosslinking with HAVE. Varied reaction times for the transesterification reaction led to different degrees of substitution, which can be used to adjust the crosslinking density of the network.
The influence of macromere content and -size of the HAVE formulations was determined by photo-rheology and an optimized formulation was found. In order to proof the biocompatibility of the newly synthesized HAVE, cells were encapsulated into the formulation by multiphoton micro¬fabrication, a high resolution 3D printing technique.
Hyaluronic acid (HA), Biocompatible hydrogels, high resolution 3D printing technique
Created from the Publication Database of the Vienna University of Technology.