[Back]


Talks and Poster Presentations (with Proceedings-Entry):

H. W. Yoo, M. E. van Royen, W. A. van Cappellen, A. B. Houtsmuller, M. Verhaegen, G. Schitter:
"Adaptive Optics for Confocal Laser Scanning Microscopy with Adjustable Pinhole";
Talk: SPIE Photonics Europe 2016, Brussels (Belgium); 04-03-2016 - 04-07-2016; in: "Proceedings of SPIE Vol. 9887, Biophotonics: Photonic Solutions for Better Health Care V", 9887 (2016), ISSN: 0967-0661; 1 - 11.



English abstract:
The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as
well as in imaging, where the size of the pinhole denotes a trade-o between out-of-focus rejection and wavefront
distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole
to cope with such a trade-o . The proposed adjustable pinhole enables to calibrate the AO system and to evaluate
the imaging performance. Experimental results with
uorescence beads on the coverslip and at a depth of 40 m
in the human hepatocellular carcinoma cell spheroid demonstrate that the proposed AO system can improve the
image quality by the proposed calibration method. The proposed pinhole intensity ratio also indicates the image
improvement by the AO correction in intensity as well as resolution.

Keywords:
Adaptive Optics, Square Adjustable Pinhole, Confocal Laser Scanning Microscopy, Pinhole Intensity Ratio

Created from the Publication Database of the Vienna University of Technology.