Talks and Poster Presentations (with Proceedings-Entry):
M. Tromayer, M. Markovic, P. Gruber, K. Hölzl, S. Vlierberghe, P. Dubruel, J. Stampfl, A. Ovsianikov, R. Liska:
"Biocompatible Two-Photon Initiator with Hyaluronan Backbone";
Poster: 4th European Symposium of Photopolymer Science,
- 09-14-2016; in: "ESPS 2016",
Two-photon polymerization (2PP) is a 3D printing approach based on femtosecond-laser-induced photopolymerization. It has been employed to produce precise 3D tissue engineering scaffolds according to computer-aided design (CAD) models. 2D substrates used in traditional cell culture systems can lead to significant differences in structure, function or physiology compared to living tissue, whereas 3D scaffolds allow a more accurate reconstruction of the cells' natural environment. Especially attractive is the option of fabrication of hydrogels with embedded cells. While specialized water-soluble two photon initiators (2PIs) have been developed, there is still a demand for novel initiators with improved cyto¬compatibility. The present work aims at this via binding 2PI molecules to hyaluronan as a polymeric backbone, thus hindering diffusion through the cell membrane and restricting the 2PI to the extracellular matrix.
A 2PI precursor was developed and covalently linked to hyaluronan to hinder migration from the extracellular matrix into cells, thus limiting cytotoxicity and photodamage by the obtained hyaluronic acid-bound photoinitiator (HAPI). Cytotoxicity testing proved superior biocompatibility of HAPI compared to reference water-soluble 2PIs. 2PP of 3D hydrogel structures was achieved by cross-linking methacrylated gelatin with HAPI. MC3T3 cells were successfully encapsulated by 2PP and viable cells were observed over a period of 5 days using a live/dead-staining assay, indicating a low phototoxicity of HAPI.
Created from the Publication Database of the Vienna University of Technology.