[Zurück]

E. Zerobin, S. Baudis, Z. Tomasikova, X.H. Qin, M. Markovic, P. Gruber, J. Stampfl, A. Ovsianikov, R. Liska:
"High Resolved Biocompatible Hydrogel Constructs based on Hyaluronic Acid";
Poster: 4th European Symposium of Photopolymer Science, Leipzig; 11.09.2016 - 14.09.2016; in: "ESPS 2016", (2016), S. P-34.

Hyaluronic acid (HA) is an FDA approved, water soluble polysaccharide in the group of glycosaminoglycanes. This natural biopolymer is widespread in the connective tissue of the human body which makes it a promising candidate for tissue engineering.
Covalent crosslinking of modified HA leads to biocompatible hydrogels. By means of introducing double bonds onto the polymer backbone, hydrogel precursor solutions are solidified by using the concept of photo-polymerization.
HA-vinylesters (HAVE)1 were obtained by transesterification reaction of the primary alcohol of the HA. Since the reactivity of vinyl esters can be increased by thiol-ene chemistry2, dithiols were used for the crosslinking with HAVE. In order to receive different degrees of substitution the reaction time of the transesterification reaction was varied.
Photo-rheological studies showed the influence of macromere content and -size of the HAVE formulations. Furthermore the crosslinking density of the hydrogels were adjusted by varying the degree of substitution.
A high resolution 3D printing technique (2-Photon-Polymerization)³ was used to encapsulate cells inside HAVE formulations to prove biocompatibility of the precursors.