Publications in Scientific Journals:
F. Baumgart, A. Arnold, K. Leskovar, K. Staszek, M. Fölser, J. Weghuber, H. Stockinger, G. Schütz:
"Varying label density allows artifact-free analysis of membrane protein nanoclusters";
Nature Methods,
13
(2016),
661
- 664.
English abstract:
We present a method to robustly discriminate clustered from randomly distributed molecules detected with techniques based on single-molecule localization microscopy, such as PALM and STORMSTORMSTORMSTORMSTORM. The approach is based on deliberate variation of labeling density, such as titration of fluorescent antibody, combined with quantitative cluster analysis, and it thereby circumvents the problem of cluster artifacts generated by overcounting of blinking fluorophores. The method was used to analyze nanocluster formation in resting and activated immune cells.
Created from the Publication Database of the Vienna University of Technology.