[Zurück]

M. Rothbauer, S. Küpcü, U. Sleytr, P. Ertl:
"Crystalline Bacterial Protein Nanolayers for Cell Micropatterning";
in: "6th European Conference of the International Federation for Medical and Biological Engineering", Springer International Publishing, 2015, S. 337 - 340.

We have developed a robust cell patterning method
using micromolding in capillaries (MIMIC) and selfassembled
crystalline bacterial protein (S-layer) monolayers.
The unique self-assembly properties of proteins SbpA and
SbsB are exploited to create an anisotropic protein nanobiointerface
with spatially-defined cytophilic (adhesive) and cytophobic
(repulsive) properties. The chosen S-layer proteins
were characterized using atomic force microscopy (AFM),
zeta-potential and contact angle measurements. Results revealed
distinct differences in surface topography and physicochemical
properties between SbsB and SbpA monolayers.
Stability testing of cytophobic protein SbpA using vacuumdrying
deposition and re-assembly on-flow sowed no remarkable
differences with respect to the antifouling properties.
Finally, using MIMIC micropatterns of epithelial CaCo-2 cell
as well as mobile Jurkat cells were e