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I. Giouroudi, S. F. Cardoso, G. Kokkinis:
"Magnetic Microfluidic Biosensor for Rapid Biomolecule Quantification";
Vortrag: EMN Hawaii Meeting, Hawaii, USA (eingeladen); 21.03.2016 - 24.03.2016; in: "Book of Abstracts EMN 2016", (2016).

In biomedical and biotechnological applications it is crucial to accurately quantify adsorbed biomolecules; for example, in order to understand the biomolecular interactions on surfaces or when working with proteins in determining a binding constant or measure enzyme kinetics. Even in cases of qualitative studies, having concrete information of how much protein is expressed enables comparative studies among experimental procedures and among proteins. Moreover, the ability to detect and quantify varying concentrations of biomarkers provides unique insight into cellular responses to external stimuli or disease pressures on an organism. The quantification of lipids is also of interest to analytical chemists when comparing lipid concentration in various functioning and diseased organisms from e.g. cancer, diabetes, hypertension, stroke etc. Another analyte gaining considerable attention is the serum auto-antibodies due to their biomedical relevance. Systemic auto-antibodies detection would facilitate the diagnostics of autoimmune disorders and evaluate their treatment and the sustained damage in organs. More interestingly the presence of certain antibodies in the system is considered an increasingly important expression of diseases such as some types of cancer. There exist several methods for measuring the amount of biomolecules usually attached to surfaces, such as ELISA, ellipsometry, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). However, these techniques are rather expensive, require highly qualified personnel and their accuracy has not yet been fully explored.

In biomedical and biotechnological applications it is crucial to accurately quantify adsorbed biomolecules; for example, in order to understand the biomolecular interactions on surfaces or when working with proteins in determining a binding constant or measure enzyme kinetics. Even in cases of qualitative studies, having concrete information of how much protein is expressed enables comparative studies among experimental procedures and among proteins. Moreover, the ability to detect and quantify varying concentrations of biomarkers provides unique insight into cellular responses to external stimuli or disease pressures on an organism. The quantification of lipids is also of interest to analytical chemists when comparing lipid concentration in various functioning and diseased organisms from e.g. cancer, diabetes, hypertension, stroke etc. Another analyte gaining considerable attention is the serum auto-antibodies due to their biomedical relevance. Systemic auto-antibodies detection would facilitate the diagnostics of autoimmune disorders and evaluate their treatment and the sustained damage in organs. More interestingly the presence of certain antibodies in the system is considered an increasingly important expression of diseases such as some types of cancer. There exist several methods for measuring the amount of biomolecules usually attached to surfaces, such as ELISA, ellipsometry, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). However, these techniques are rather expensive, require highly qualified personnel and their accuracy has not yet been fully explored.