Publications in Scientific Journals:
M. Tromayer, P. Gruber, M. Markovic, A. Rosspeintner, E. Vauthey, H. Redl, A. Ovsianikov, R. Liska:
"A biocompatible macromolecular two-photon initiator based on hyaluronan";
The possibility of the direct encapsulation of living cells via two-photon induced photopolymn. enables the microfabrication of hydrogel scaffolds with high initial cell loadings and intimate matrix-cell contact. While highly efficient water-sol. two-photon initiators based on benzylidene ketone dyes have been developed, they exhibit considerable cyto- and phototoxicity. To address the problem of photoinitiator migration from the extracellular matrix into the cytosol, a two-photon initiator bound to a polymeric hyaluronan backbone (HAPI) was synthesized in this work. HAPI exhibited a distinct improvement of cytocompatibility compared to a ref. two-photon initiator. Basic photophys. investigations were performed to characterize the absorption and fluorescence behavior of HAPI. Laser scanning microscopy was used to visualize and confirm the hindered transmembrane migration behavior of HAPI. The performance of HAPI was tested in two-photon polymn. at exceedingly high printing speeds of 100 mm s-1 producing gelatin-based complex 3D hydrogel scaffolds with a water content of 85%. The photodamage of the structuring process was low and viable MC3T3 cells embedded in the gel were monitored for several days after structuring.
two-photon initiator based on hyaluronan, the direct encapsulation of living cells, photopolymerisation, Biocompatibility, Macromolecular compounds,
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
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Created from the Publication Database of the Vienna University of Technology.