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S. Helfert, C. Dworak, E. Sevcsik, H. Peterlik, M. Sauer, D. Ret, R. Liska:
"Synthesis and Characterization of Polymer Linker systems for T-cell activation";
Poster: 12th International Conference on Advanced Polymers via Macromolecular Engineering, Ghent; 21.05.2017 - 25.05.2017; in: "Advanced Polymers via Macromolecular Engineering", (2017), S. 133.

In the field of biomedicine specifically designed polymer brush systems gained much interest due to their ability to control a number of important architectural features1 for the creation of particular biointerfaces2-4 and applications in nanotechnologies on different substrates4-6.
In this study it was of special interest to prepare a polymer linker system to glass substrates for monitoring of specific cell interactions via total internal reflection microscopy. Thus, uniform polymer brushes were prepared using reversible addition fragmentation chain transfer polymerization (RAFT), which facilitates a polymerization under gentle temperatures, without toxic catalysts, providing low dispersity and defined molecular weights. Poly-(N-acryloylmorpholine) and poly-(N-(3-methoxypropyl)acrylamide) polymers generated by RAFT with a MW of approximately 20 kDa were investigated via SLS, DLS and SAXS to determine their size, shape and structural stability in aqueous environments. The results of these measurements showed, that strong aggregation occurred in aqueous solution, and that the formed aggregates exhibited a Kholodenko-worm-like shape in water and PBS, indicating potential suitability for the targeted polymer brush system. Further approaches for grafting of polymers from glass substrates were analyzed via contact angle and ellipsometry.

biomedicine specifically designed polymer brush systems, reversible addition fragmentation chain transfer polymerization (RAFT), Poly-(N-acryloylmorpholine) and poly-(N-(3-methoxypropyl)acrylamide) polymers