[Zurück]

S. Baudis, E. Zerobin, M. Lunzer, M. Tromayer, M. Markovic, P. Gruber, J. Stampfl, A. Ovsianikov, R. Liska:
"Controlling the Micro-Environment of Cells by Exploitation of Macromolecular Photochemistry";
Vortrag: 12th International Conference on Advanced Polymers via Macromolecular Engineering, Ghent; 21.05.2017 - 25.05.2017; in: "Advanced Polymers via Macromolecular Engineering", (2017), S. 91.

Multi-photon-processes are nonlinear optical
phenomena only occuning at high photon densities in
the focus of femtosecond pulsed lasers thus can trigger
photochemistry within confined space (< 1 ¡rm) inside
materials. We present a toolbox of biocompatible
photochemistry enabling the manipulation of the
micro-environment of living cells. Employing
two-photon-iniriarors (2pI) with either
photo-crosslinking or photo-degrading systems
enables cell-photoencapsulation or photo_milling of
channels for directed cell growth, respectively, within
e two-photonJithography device. Materials of choice
are hydrogels with precursors based on natural (e.g.,
gelatin, hyaluronan, etc.) or biocompatible synthetic
polymers (e.g. poly(vinyl alcohol)). These precursors
were modified with enes (e.g., methacrylates,
vinylesters, norbornenes) or thiols and combined with
other (macromolecular) crosslinkers in thiol-ene
coupling reactions to form hydroget networks with
arbitrary shape in high resolution. Incorporation of
photo-labile groups into networks enables the
photo-cleavage of formed crosslinks by the
two-photon-process. Taken together these material
platforms accompanied by two-photon-technology
allow for a spatial-temporal conirol of the hydrogel
properties in the presence of living cells and are
therefore an ideal platform technology to study the
behavior of cells in different micro-environments.

Multi-photon-processes, photochemistry, biocompatible photochemistry, micro-environment of living cells, hydrogels with precursors based on natural or biocompatible synthetic polymers , behavior of cells in different micro-environments

http://publik.tuwien.ac.at/files/publik_260739.pdf