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S. Helfert, C. Dworak, E. Sevcsik, H. Peterlik, D. Ret, M. Sauer, R. Liska:
"Synthesis and Characterization of Polymer Linker Systems for Cell Studies";
Vortrag: Bypos Workshop 2017, Zemplínská Sírava, Slovakia; 12.06.2017 - 16.06.2017; in: "Bypos Workshop Book 2017", (2017), S. 29.

The generation and design of polymer brushes became very attractive in the field of biomedical applications. Their ability to control a number of important architectural features makes them a powerful tool in the creation of particular biointerfaces and applications in nanotechnologies on a variety of substrates.1 In this study, a strategy for a polymer linker system on SiO2 substrates was established, designed for the coupling to biomolecules. For the implementation of this polymer brush system, surface-induced reversible addition fragmentation chain transfer (RAFT) polymerization was chosen, delivering gentle polymerization conditions, absence of toxic catalysts, a variety of possible end-group modifications, uniform chain lengths and defined molecular weights. Poly-(N-acryloylmorpholine) and poly-(N-(3-methoxypropyl)acrylamide) were generated via RAFT polymerization with a MW of approximately 20 kDa. Their morphology and behavior in aqueous solutions was investigated via light scattering methods (SLS, DLS) and small angle X-ray scattering (SAXS) to predict their arrangement in a polymer brush system. The results of these measurements showed worm-like aggregation in aqueous environments. Further investigations were performed on end-group formation of these RAFT polymers and showed good results for radical induced end-group formation. Different approaches for the immobilization of a RAFT reagent on SiO2 surfaces were performed, using aminopropyl triethoxysilane (APTES), for grafting of polymers from the substrate and were analyzed via contact angle and ellipsometry.

polymer brushes, biomedical applications, nanotechnologies, polymer linker system on SiO2 substrates